The National Institute of Allergy and Infectious Diseases (NIAID) is an institute within the National Institutes of Health that leads research to understand, treat, and prevent infectious, immunologic, and allergic diseases. In support of this mission, NIAID desired to revert Room 2E13B into a Tissue Culture lab which will be used for critical COVID-19 research. The new space included the installation of Mass Spectrometer equipment and other laboratory equipment provided by NIH, so the space had to be designed to meet the manufacturer’s requirements. The lab design met the NIAID end user requirements and the standards outlined in the NIH Design Requirements Manual (DRM). Architectural scope of work included repainting all walls to match existing, replacing all damaged acoustical ceiling tiles, and stripping and waxing the existing floor. Millwork included constructing a new fixed laboratory bench with solid surface countertop and built-in sink at the same location of the existing sink. Laboratory bench had base cabinets and a storage cabinet below the sink. The project also included requirements for metal fabrications, penetration firestopping, joint sealants, hollow metal doors and frames, glazing, non-structural metal framing, gypsum board, acoustical panel ceilings, and high-performance coatings. Furnishing’s scope of work included installation of metal laboratory casework. Plumbing scope of work included adding an extension to the existing CO2 line located in the wall in the laboratory. Matos connected the new sink that will be built into the new Laboratory bench. Matos connected two NIH furnished Bio-Safety Cabinets to the existing Vacuum line that was currently located in the ceiling. The project also included requirements for drainage of vent systems, plumbing fixtures, and compressed gas systems. Mechanical scope of work included reviewing the NIH end-user equipment list and verifying that the existing HVAC in the room was sufficient. Equipment included: (1) NIH provided two 4’ Biosafety Cabinets (Bio-Safety Cabinet-Class II Type A2), Nuaire Labgard Energy Saver NU-540-400 Biosafety Cabinet, (2) Thermo Fisher Scientific Heracell VIOS 160i Copper CO2 incubator. There were two stacks of two incubators (total of four incubators), (3) Thermo Scientific Sorvall X4R Pro-MD, 120V Refrigerated centrifuge, (4) Thermo Scientific™ Precision™ General Purpose Baths 5L, (5) VWR® Analog Vortex Mixer, (6) Thermo Scientific Sorvall Legend Micro 17R Refrigerated Micro Centrifuge, (7) Roche MagNA Lyser, (8) Inverted Microscope, and (9) Waters BioAccord Mass Spectrometer. Matos ensured that the space was designed to meet the following condition requirements for the BioAccord System: (1) The ambient temperature range required for operation is 15 to 28 °C (59 to 82.4 °F), (2) The relative humidity in which the instrument and pumps operate must be in the range of 20% to 80%, noncondensing. Matos provided a new ventilation design for the BioAccord-Mass Spectrometer System and provided Pre-Construction and Post-Construction Testing and Air Balancing Report. The projectincluded demolishing and caping two runs of ceiling mounted flexible ductwork, and existing laboratory sink. The project also included requirements for basic mechanical requirements, mechanical insulation, F4 (fiberglass with ASJ and PVC jacket, fiberglass insulation thickness (FTHK Chart), piping materials and methods, piping systems, CU01 (General Copper Type L Tubing), CU02 (Oxygen Use Copper Type L Tubing), PP31 (Polypropylene Fire-Resistant Waste System), metal ductwork, testing/adjusting and balancing. Electrical scope of work included reviewing the NIH end-user equipment list and verifying that the existing power in the room was sufficient. Matos provided thirty days metering of electrical panel serving Room 2E13B – Tissue Culture lab. Matos also furnished and installed one L6-30R, 208V, 30 Amp duplex power outlet that was connected to the building UPS. The project also included requirements for basic electrical requirements, low-voltage electrical power conductors and cables, grounding and bonding for electrical systems, raceways, electrical boxes and fittings, and wiring devices. Electronic Safety and Security scope of work included requirements for fire detection and alarm systems.
